Hematological Disease Studies – FISH Panels and Specific Probes

Clinical info FISH analysis is available to rule out certain common oncology related translocations, deletions and amplifications. This analysis is particularly useful when a specific hematologic disease is highly suspected (i.e. Philadelphia chromosome in chronic myelogenous leukemia) and/or cells fail to grow in culture. FISH can be used to look for minimal residual disease in patients undergoing treatment or in patients thought to be going into or coming out of remission. It can be used to detect unusual variants of the Philadelphia chromosome translocation, and to follow bone marrow transplants in certain patients. Since most of these oncology probes are used on interphase cells, standard cytogenetics is still necessary to look for other chromosomal aberrations and to detect clonal evolution of disease, an important prognostic indicator.
Single Gene Probes (deletion or amplification) P58 CLK-1 Locus (1p36)
D7S486 (7q31)
Retinoblastoma (13q14)
p53 (17p13.1)
Her-2/neu (17q11.2-q12)
Enumeration probes for all chromosomes Dual Color Translocation Probes
bcr/abl translocation t(9;22)(q34;q11.2) (both major and minor breakpoints)
M-bcr/abl translocation t(9;22)(q34;q11.2) (major breakpoint)
IGH/CCND1 translocation t(11;14)(q13;q32)
PML/RARA translocation t(15;17)(q22;q21.1)
TEL/AML1 translocation t(12;21)(p13;q22)
FISH Hematology Disorder Panels Acute Lymphocytic Leukemia
Acute Myelocytic Leukemia
Burkitt’s Lymphoma
Chronic Lymphocytic Leukemia
Chronic Myelocytic Leukemia
Chronic Myelomonocytic Leukemia
Multiple Myeloma
Myelodysplastic states
Non-Hodgkin Lymphoma
Pediatric ALL
Translocation Probes FGFR3/IgH t(4;14)
IgH/MYC/CEP 8 t(8;14)
AML1/ETO t(8;21)
BCR/ABL t(9;22)
IgH/CCND1 t(11;14)
TEL(ETV6)/AML1 t(12;21)
IgH/MAF t(14;16)
PML/RARA t(15;17)
IgH/MALT1 t(14;18)
Individual Probes (Interphase and/or Metaphase)
  • MYC (2p24.1)
  • ALK (2p23)
  • BCL6 (3q27)
  • CSF1R (5q33-q34)
  • EGR-I (5q31)
  • MYB (6q23)
  • EGFR (7p12)
  • D7S486 (7q31)
  • C-MYC (8q24)
  • p16 (9p21)
  • 9q34
  • PTEN (10q23)
  • Cyclin D1/CCND1 (11q13)
  • ATM (11q22.3)
  • MLL (11q3)
  • ETV6/TEL (12p13)
  • Retinoblastoma (RB1)
  • D13S319 (13q14)
  • IgH (14q32)
  • CBFB (inv16)
  • p53 (17p13.1)
  • TOP2A (17q21)
  • SYT (18q11.2)
  • MALT1 (18q21)
  • BCL2 (18q21)
  • D20S108 (20q12)
  • 20q13
  • EWSR1 (22q12)
Specimen Requirements FISH can be performed on any tissue that can be cultured for chromosome analysis and interphase FISH can be performed on any cytogenetic sample. Follow collection and transport guidelines specific for each tissue type. Studies requested should be indicated at the time of sample submission.
Transport Specimen should be kept at room temperature; do not freeze or refrigerate. Specimen should be sent by courier or overnight mail to arrive at the laboratory within 24 hours.
Turnaround time 21 days
FISH on Hematologic/Oncology studies Price CPT Codes
ALL Screen (interphase) t(9;22), t(8;14), t(12;21), MLL rearrangements, trisomy 8, 9p21, 11q23 $1640.00 88275 (x2), 88271 (x9), 88291
AML Screen (interphase) t(8;21), t(15;17), MLL rearrangements, inv 16, t(9;22), trisomy 8, 11q23, monosomy 7, del5q/monosomy 5, delp53, t(12;21) $1766.00 88275 (x2), 88271 (x10), 82291
Multiple myeloma screen (interphase) del 13q (RB1 and D13S319), trisomy 9, IgH rearrangements, p53, t(4;14), t(14;16), del6q23 $1010.00 88275 (x2), 88271 (x4), 88291
Myelodysplastic States Screen (interphase) -5/5q-, -7/7q-, trisomy 8, X and Y, t(9;22), 11q23, del20q12 $1262.00 88275 (x2), 88271 (x6), 88291
Non-Hodgkins Lymphoma Screen t(14;18), t(8;14), del p53, trisomy 8, trisomy12 $1262.00 88275 (x2), 88271 (x6), 88291
Pediatric ALL Screen (interphase) t(9;22),trisomy 4, 10, 17, t(12;21) $1388.00 88275 (x2), 88271 (x7), 88291
Chromic Lymphocytic Leukemia Screen del p53, del 13q (RB1 and D13S319), trisomy 12 $1034.00 88275, 88271 (x6), 88291
Chronic Myelocytic Leukemia t(9;22) [BCR/ABL/9q34], Trisomy 8 [Classical 8 $782.00 88275, 88271 (x4), 88291
Burkett’s Lymphoma (MYC[MYC DCBA]) $404.00 88275, 88271, 88291
CMML ( 12p13 [ETV6]) $404.00 88275, 88271, 88291

For Hematologic samples if no chromosomes are requested, $240 culture fee (88230)
For Oncology samples if no chromosomes are requested, $300 culture fee (88237)

Contact For further information contact one of our This email address is being protected from spambots. You need JavaScript enabled to view it. at 1-800-473-9411.

Cytogenetics Lab

The Cytogenetics Lab provides high quality analysis for the detection of both constitutional chromosomal aberrations as well as abnormalities related to hematological malignancies

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