Chromosome Analysis, Routine; Rule Out Mosaic (Amniotic Fluid)

Chromosome mosaicism is defined as the presence of two or more cell lines with different chromosome constitutions in a single individual. Chromosome analysis to rule out mosaicism includes routine karyotyping using G-banded preparations, but additional cells are counted compared to routine chromosome analysis. A minimum of 50 cells are counted and 5 cells are analyzed.

Turnaround Time

14 days

CPT Code(s)

88235, 88263, 88285 x5, 88291

Cost

$827

This test can be performed from direct amniotic fluid or on cultured amniocytes. If sending direct fluid for microarray only, 10-20 ml of amniotic fluid is requested. Chromosomes and FISH will require an additional 10-15 ml of fluid. If sending cultured flasks, 2x T25 confluent flasks are required. Parental samples are recommended to accompany prenatal specimen. 4-5 ml of peripheral blood should be collected on each parent in an EDTA (lavender top) tube. Additional specimen types include: saliva and extracted DNA.

Amniotic fluid should be kept at room temperature; do not freeze or refrigerate. Specimen should be sent by courier or overnight mail to arrive at the laboratory the next day.