Pregnancy Loss Microarray

The pregnancy loss microarray is performed using either Affymetrix CytoScan HD or OncoScan. This analysis can detect abnormalities such as aneuploidy, mosaicism as low as 20%, common microdeletion and microduplication syndromes, interstitial and terminal chromosome deletions and duplications greater than >300 kb, and loss of heterozygosity and suspected uniparental disomy (UPD).

Turnaround Time

26 days

CPT Code(s)

81229

Cost

$1,950

Pregnancy loss occurs in up to 15% of all recognized pregnancies. In approximately half of these cases, the loss can be attributed to the presence of one or more chromosome abnormalities. The pregnancy loss microarray is performed using either Affymetrix CytoScan HD or OncoScan, and the appropriate platform will be determined by sample type and condition. This analysis can detect the following abnormalities: Aneuploidy Mosaicism as low as 20% Common microdeletion and microduplication syndromes Interstitial and terminal chromosome deletions and duplications greater than >300 kb Loss of heterozygosity and suspected uniparental disomy (UPD) If a maternal sample is also sent to the laboratory, maternal cell contamination can be detected, and the parent of origin for uniparental isodisomy can be determined.

Pregnancy loss microarray can be performed on fetal samples following miscarriage, fetal demise, or stillbirth.

Fresh tissue (fetal tissue, amnion, placenta) Cultured cells Formalin fixed paraffin embedded (FFPE) Products of Conception should be placed in a sterile container for transport. The specimen must be kept moist, so add tissue culture media or sterile saline if needed. Fresh tissue: Using sterile technique, obtain a 5 mm biopsy of unmacerated fetal tissue and place in tube containing transport media. If tissue culture media is not available, sterile solutions such as balanced salt solution may be used. The preferred placental tissues are fetal membranes or chorionic villi. If fetal samples are obtained at autopsy, lung, gonad or thymus are preferred for chromosome study. Cultured tissue: 2-T25 confluent flasks can be sent for analysis. Extracted DNA is also accepted for this test. Please note that a maternal sample (4-5 ml of blood collected in a lavender-top/EDTA tube) is strongly recommended to rule out the presence of maternal cell contamination in POC samples. Additional specimen types include: saliva and extracted DNA. An additional charge ($350) will apply.

The specimen should be kept at room temperature and delivered via overnight shipping. Do not freeze.