Chromosome Analysis, Routine; Rule Out Mosaic (POC)
Key Information
Lab:
TAT:
6 weeks
Price:
$857
CPT Code(s):
88233, 88263, 88285x5, 88291
Test Code:
CPLM
Chromosome Analysis, Routine; Rule Out Mosaic (POC)
Chromosome mosaicism is defined as the presence of two or more cell lines with different chromosome constitutions in a single individual. Chromosome analysis to rule out mosaicism from solid tissue includes routine karyotyping using G-banded preparations. This test also detects chromosome abnormalities at a larger count to assess for mosaicism.
Please note that additional fees may apply if more than one tissue type is submitted. Please use a multiplier equal to the number of samples submitted for CPT Code 88233.
Clinical Information
Chromosome analysis on products of conception can help clarify if an atypical number of chromosomes might have contributed to a miscarriage, fetal demise, or stillbirth.
Technical Information
For rule out mosaic chromosome analysis, 50 cells are counted to determine the modal number and a minimum of 5 cells are analyzed for chromosomal abnormalities from G-banded preparations.
Specimen Requirements
Using a sterile technique, obtain POC and place in a sterile transport container with tissue transport media or a sterile solution such as a balanced salt solution. DO NOT allow the specimen to come into contact with formalin or other fixatives if chromosome analysis is ordered as fixed tissue will not grow for chromosome analysis. Chromosome analysis cannot be performed on FFPE samples.
A maternal sample is recommended to accompany the POC sample for maternal cell contamination (MCC) studies. The preferred maternal sample type for MCC is 3-4ml of peripheral blood collected in an EDTA (purple top) tube. Additional maternal sample types include extracted DNA or saliva.
Send approximately 5µg of extracted DNA at a requested concentration of 90-130 ng/µl.
Saliva samples must be submitted in an approved saliva kit.
Blood and saliva kits are available by request.
Transport Instructions
POC should be should kept at room temperature if transported immediately. If the specimen is not being immediately transported to the laboratory, it may be refrigerated. Do not freeze. The sample should be sent by courier or overnight mail to arrive at the laboratory within 24 hours.
The blood sample for MCC should be kept at room temperature and delivered via overnight shipping. If shipment is delayed by one or two days, the specimen should be refrigerated and shipped at room temperature. Do not freeze the specimen. Samples collected on Friday can be safely designated for Monday delivery.
Extracted DNA should be sent at room temperature via overnight delivery.
Saliva is stable at room temperature and can be delivered via overnight or ground shipping.
Associated Tests
Connect With Our Experts
Call 1-800-473-9411 to speak with our team of laboratory genetic counselors for questions or additional information.
Robin Fletcher, MS, CGC
Falecia Thomas, MS, CGC
Alex Finley, MS, CGC