Chromosome Analysis (Stimulated/Unstimulated Blood)
Key Information
Lab:
TAT:
10 days
Price:
$890
CPT Code(s):
88237, 88264, 88280x2, 88291
Test Code:
CBSU
Chromosome Analysis (Stimulated/Unstimulated Blood)
Chromosome analysis on stimulated/unstimulated cultures can be used to assess remission or relapse of malignancy and/or to determine the presence or absence of abnormal clones. The finding of a normal karyotype is generally considered to be a favorable prognostic indicator. Submission of a follow-up specimen to monitor karyotypic evolution should be considered as clinically permitted or as the clinical course changes.
Clinical Information
Chromosome analysis on stimulated/unstimulated cultures is helpful in assessing remission or relapse of malignancy and/or to determine the presence or absence of abnormal clones. The finding of a normal karyotype is generally considered to be a favorable prognostic indicator. Submission of a follow-up specimen to monitor karyotypic evolution should be considered as clinically permitted or as the clinical course changes.
Technical Information
Stimulated and unstimulated cultures are prepared. The karyotype of spontaneously dividing (unstimulated) cells will be similar to that obtained from bone marrow. In contrast, stimulated cultures are treated with a mitogen to stimulate division of normal lymphoid cells. Stimulation of normal cells may interfere with the analysis of spontaneously dividing malignant cells. Therefore, analysis of stimulated cultures will detect abnormalities in the constitutional karyotype but will not generally detected chromosomal abnormalities related to hematological malignancy. GTW banding is performed on metaphase spreads. The karyotyping of metaphases is performed using the Leica CytoVision system.
Specimen Requirements
The accepted sample type is 1-5ml of whole blood collected in a sodium heparin vacutainer.
Transport Instructions
Whole blood should be kept at room temperature and sent by courier or overnight shipping to arrive at the laboratory within 24 hours. Do not freeze or refrigerate the specimen.
Associated Tests
Connect With Our Experts
Call 1-800-473-9411 to speak with our team of laboratory genetic counselors for questions or additional information.
Robin Fletcher, MS, CGC
Falecia Thomas, MS, CGC
Alex Finley, MS, CGC